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M3546
Sigma
Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)
Description
| Application | The ultralow range marker is designed for use in the Tris-Tricine SDS-PAGE method when stained with Brilliant blue G (non-methanol based). Glutaraldehyde fixing is recommended with the ultralow marker in order to keep the two smallest proteins fixed in the gel. The ultralow range marker is supplied as a liquid, which must be diluted (1:20) with the supplied sample buffer prior to loading. |
| Physical form | Solution in 0.1 M Tris-HCl, pH approx. 8.5, with 4 mM EDTA, 3 mM sodium azide and 40% glycerol |
Properties
| usage | vial sufficient for 400 applications on large gels (16 × 18 cm) |
| vial sufficient for 800 mini-gels (10 × 10 cm) | |
| packaging | vial of 200 μL |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
Components
| Standard component | Triose Phosphate Isomerase, Rabbit Muscle 26,600 Da MW |
| Myoglobin, Horse Heart, 17,000 Da MW | |
| α-Lactalbumin, Bovine Milk, 14,200 Da MW | |
| Aprotinin, Bovine Lung, 6,500 Da MW | |
| Insulin Chain B, Oxidized, Bovine, 3,496 Da MW | |
| Bradykinin, 1,060 Da MW | |
| Tris-Tricine sample buffer 10 mL |
References
| reference | Schagger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379, (1987) Abstract |
